![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800041:Design
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-PatA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4036
Illegal PstI site found at 4048 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4048
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4030
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4036
Illegal PstI site found at 4048 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4036
Illegal PstI site found at 4048
Illegal NgoMIV site found at 2804
Illegal AgeI site found at 3311 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4232
Illegal BsaI site found at 5300
Illegal SapI.rc site found at 767
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
None